confocor2 fcs unit (Carl Zeiss)

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Carl Zeiss confocor2 fcs unit
Confocor2 Fcs Unit, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocor2+fcs+system/confocor2+fcs+system/pm37640758-143-7-18
Average 90 stars, based on 1 article reviews

confocor2 fcs unit - by Bioz Stars, 2026-09

90/100 stars

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Diffusion-based Assay:

Article Title: Interleukin-7 Compartmentalizes Its Receptor Signaling Complex to Initiate CD4 T Lymphocyte Response
Article Snippet: For cytokine activation, CD4 T cells were resuspended at 10 6 cells/ml in RPMI 1640 medium (Lonza, Verviers, Belgium) supplemented with 5% fetal bovine serum, 50 m m HEPES, pH 7.4, glutamine, penicillin, streptomycin, and fungizone (complete medium) in 24-well plates, were treated with 1 n m recombinant glycosylated human IL-7 (Cytheris) at 37 °C in a 5% CO 2 humidified atmosphere. .. FCS and FCCS Analysis of IL-7R Chain Assembly and Diffusion at the Surface of Living Cells Fluorescence auto- and cross-correlated spectroscopy (FCS/FCCS) measurements were made on living cells using an inverted laser scanning confocal microscope (LSM510), combined with a ConfoCor2 FCS system (Zeiss). .. Depth of field was spatially filtered through a 30–300-μm pinhole and fluorescence light was split into two detection channels with the following excitation/emission wavelengths: Alexa Fluor 488 (Invitrogen) (Ar, 488 nm/550–600 nm) and Alexa Fluor 633 (Invitrogen) (He/Ne, 633 nm/BP680 nm).

Article Title: IL-2 induces conformational changes in its preassembled receptor core, which then migrates in lipid raft and binds to the cytoskeleton meshwork.
Article Snippet: *Corresponding author.. Unité d'Im 15, France.. E-mail address: rose@pa † A.-H.P. and V.L. contributed eq Present address: F. Gesbert, INSE André Lwoff, F-94807 Villejuif, Fran Abbreviations used: IL, interleuki monoclonal antibody; FCS, fluoresce GFP, green fluorescent protein; ECF ROI, region(s) of interest; PFA, para dimensional; 2D, two-dimensional; C mAbb, biotinylated monoclonal anti DRM, detergent-resistant membrane TBS, Tris-buffered saline; PDB, Prot

Article Title: Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL-7 Receptor Signalosome in Human CD4 T-cells
Article Snippet: Cells were mounted in Vectashield/DAPI medium (Vector Laboratories) for confocal microscopy and in Mowiol (4-88; Sigma) supplemented with antifading Dabco (35 mg/ml; Sigma) for STED microscopy. .. Receptor Chain Diffusion Rate Analysis by Fluorescence Cross-correlated Spectroscopy All protein diffusions at the surface of living human CD4 T-cells were acquired and analyzed in the absence and presence of raft and cytoskeleton inhibitors by spot variation FCS ( 16 ) using an inverted laser-scanning confocal microscope (LSM510, Zeiss), combined with a ConfoCor2 FCS system (Zeiss), as described previously ( 10 ), and detailed in the supplemental material . ..

Article Title: Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL-7 Receptor Signalosome in Human CD4 T-cells
Article Snippet: Cells were mounted in Vectashield/DAPI medium (Vector Laboratories) for confocal microscopy and in Mowiol (4-88; Sigma) supplemented with antifading Dabco (35 mg/ml; Sigma) for STEDmicroscopy. .. Receptor Chain Diffusion Rate Analysis by Fluorescence Cross-correlated Spectroscopy—All protein diffusions at the surface of living human CD4 T-cells were acquired and analyzed in the absence and presence of raft and cytoskeleton inhibitors by spot variation FCS (16) using an inverted laserscanning confocalmicroscope (LSM510, Zeiss), combinedwith a ConfoCor2 FCS system (Zeiss), as described previously (10), and detailed in the supplemental material. ..

Fluorescence:

Article Title: Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL-7 Receptor Signalosome in Human CD4 T-cells
Article Snippet: Cells were mounted in Vectashield/DAPI medium (Vector Laboratories) for confocal microscopy and in Mowiol (4-88; Sigma) supplemented with antifading Dabco (35 mg/ml; Sigma) for STEDmicroscopy. .. Receptor Chain Diffusion Rate Analysis by Fluorescence Cross-correlated Spectroscopy—All protein diffusions at the surface of living human CD4 T-cells were acquired and analyzed in the absence and presence of raft and cytoskeleton inhibitors by spot variation FCS (16) using an inverted laserscanning confocalmicroscope (LSM510, Zeiss), combinedwith a ConfoCor2 FCS system (Zeiss), as described previously (10), and detailed in the supplemental material. ..

Article Title: Interleukin-7 Compartmentalizes Its Receptor Signaling Complex to Initiate CD4 T Lymphocyte Response
Article Snippet: For cytokine activation, CD4 T cells were resuspended at 10 6 cells/ml in RPMI 1640 medium (Lonza, Verviers, Belgium) supplemented with 5% fetal bovine serum, 50 m m HEPES, pH 7.4, glutamine, penicillin, streptomycin, and fungizone (complete medium) in 24-well plates, were treated with 1 n m recombinant glycosylated human IL-7 (Cytheris) at 37 °C in a 5% CO 2 humidified atmosphere. .. Fluorescence auto- and cross-correlated spectroscopy (FCS/FCCS) measurements were made on living cells using an inverted laser scanning confocal microscope (LSM510), combined with a ConfoCor2 FCS system (Zeiss). .. Depth of field was spatially filtered through a 30–300-μm pinhole and fluorescence light was split into two detection channels with the following excitation/emission wavelengths: Alexa Fluor 488 (Invitrogen) (Ar, 488 nm/550–600 nm) and Alexa Fluor 633 (Invitrogen) (He/Ne, 633 nm/BP680 nm).

Spectroscopy:

Article Title: Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL-7 Receptor Signalosome in Human CD4 T-cells
Article Snippet: Cells were mounted in Vectashield/DAPI medium (Vector Laboratories) for confocal microscopy and in Mowiol (4-88; Sigma) supplemented with antifading Dabco (35 mg/ml; Sigma) for STEDmicroscopy. .. Receptor Chain Diffusion Rate Analysis by Fluorescence Cross-correlated Spectroscopy—All protein diffusions at the surface of living human CD4 T-cells were acquired and analyzed in the absence and presence of raft and cytoskeleton inhibitors by spot variation FCS (16) using an inverted laserscanning confocalmicroscope (LSM510, Zeiss), combinedwith a ConfoCor2 FCS system (Zeiss), as described previously (10), and detailed in the supplemental material. ..

Article Title: Interleukin-7 Compartmentalizes Its Receptor Signaling Complex to Initiate CD4 T Lymphocyte Response
Article Snippet: For cytokine activation, CD4 T cells were resuspended at 10 6 cells/ml in RPMI 1640 medium (Lonza, Verviers, Belgium) supplemented with 5% fetal bovine serum, 50 m m HEPES, pH 7.4, glutamine, penicillin, streptomycin, and fungizone (complete medium) in 24-well plates, were treated with 1 n m recombinant glycosylated human IL-7 (Cytheris) at 37 °C in a 5% CO 2 humidified atmosphere. .. Fluorescence auto- and cross-correlated spectroscopy (FCS/FCCS) measurements were made on living cells using an inverted laser scanning confocal microscope (LSM510), combined with a ConfoCor2 FCS system (Zeiss). .. Depth of field was spatially filtered through a 30–300-μm pinhole and fluorescence light was split into two detection channels with the following excitation/emission wavelengths: Alexa Fluor 488 (Invitrogen) (Ar, 488 nm/550–600 nm) and Alexa Fluor 633 (Invitrogen) (He/Ne, 633 nm/BP680 nm).

Microscopy:

Article Title: Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL-7 Receptor Signalosome in Human CD4 T-cells
Article Snippet: Cells were mounted in Vectashield/DAPI medium (Vector Laboratories) for confocal microscopy and in Mowiol (4-88; Sigma) supplemented with antifading Dabco (35 mg/ml; Sigma) for STED microscopy. .. All protein diffusions at the surface of living human CD4 T-cells were acquired and analyzed in the absence and presence of raft and cytoskeleton inhibitors by spot variation FCS ( 16 ) using an inverted laser-scanning confocal microscope (LSM510, Zeiss), combined with a ConfoCor2 FCS system (Zeiss), as described previously ( 10 ), and detailed in the supplemental material . ..

Article Title: The stratified syncytium of the vertebrate lens
Article Snippet: .. Lenses from tamoxifen-treated Cre-ERTM;Z/EG mice were viewed using an LSM510 meta confocal microscope equipped with a Confocor2 FCS system (Carl Zeiss, Thornwood, NY). ..

Article Title: Interleukin-7 Compartmentalizes Its Receptor Signaling Complex to Initiate CD4 T Lymphocyte Response
Article Snippet: For cytokine activation, CD4 T cells were resuspended at 10 6 cells/ml in RPMI 1640 medium (Lonza, Verviers, Belgium) supplemented with 5% fetal bovine serum, 50 m m HEPES, pH 7.4, glutamine, penicillin, streptomycin, and fungizone (complete medium) in 24-well plates, were treated with 1 n m recombinant glycosylated human IL-7 (Cytheris) at 37 °C in a 5% CO 2 humidified atmosphere. .. Fluorescence auto- and cross-correlated spectroscopy (FCS/FCCS) measurements were made on living cells using an inverted laser scanning confocal microscope (LSM510), combined with a ConfoCor2 FCS system (Zeiss). .. Depth of field was spatially filtered through a 30–300-μm pinhole and fluorescence light was split into two detection channels with the following excitation/emission wavelengths: Alexa Fluor 488 (Invitrogen) (Ar, 488 nm/550–600 nm) and Alexa Fluor 633 (Invitrogen) (He/Ne, 633 nm/BP680 nm).

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